Adaptation of the method for identification and quantification of sinigrin in mustard seeds

The research is devoted to the study on adaptation, verification and implementation of method for determining sinigrin in mustard seeds products its processing, as well other types food raw materials plant origin. relevance determined, one hand, by need control technological processes and, lack effective standardized methods Russian Federation. aim modify (or) adapt most identification quantification sinigrin, presented publications foreign researchers. objectives included construction calibration graphs; modification sample preparation technique reduce complexity duration analysis while maintaining required parameters accuracy reproducibility; adaptation using high performance liquid chromatography; approbation proposed real production samples, i.e. seeds.High chromatography has been used basic method.As a result research, modified. It made it possible labor intensity time metrological characteristics. dependence determined quantitative origin high-performance chromatograph equipped with diode array mass spectrometric detectors proposed. As verification, characteristics have determined: measurement range from 0.0005 10%, index ± 0.01%. tested objects, seeds, which are main sources sinigrin. can be recommended use laboratories necessary measuring instruments testing equipment.